Cell Specification
Vascular smooth muscle cell is the cellular substrate of most significant arterial diseases[1]. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate vascular smooth muscle cells expression ICAM-1 and VCAM-1 which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular diseases [2]. In vitro culture of human VSMC as a model of vascular research has played a critical role and continue to provide information in the pharmacology and therapy of vascular diseases.

HBVSMC from ScienCell Research Laboratories are isolated from human brain vascular tissue. HBVSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBVSMC are characterized by immunofluorescent method with antibodies to alpha-smooth muscle actin and desmin. HBVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBVSMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. No. 1101) for the culturing of HBVSMC in vitro.

Product Use
HBVSMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444.
[2] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401.