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Human Choroid Plexus Endothelial Cells
(HCPEC)
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| Catalog Number: 1300 |
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Cell Specification
The choroid plexus plays a wide range of roles in brain development,
maturation, aging process, endocrine regulation, and pathogenesis
of certain neurodegenerative diseases [1]. The choroid plexuses
consist of a single layer of epithelial cells enclosing a vascular
core that together form the blood-cerebrospinal fluid barrier [2].
The choroidal capillary is a single layer of endothelial cells interrupted
by "pores" which exhibit a diaphragm between the lumen and the interstitial
space. Study shows that choroid plexus endothelial cells express
high Glut1 glucose transporter [3]. The high glucose transport densities
in choroids plexus endothelial cells is consistent with the suggestion
that choroids epithelial and endothelial cells provide a metabolic
work capability for maintaining ionic gradients and secretory functions
across the blood-cerebrospinal fluid barriers.
HCPEC from ScienCell Research Laboratories are isolated from human
choroid plexus. HCPEC are cryopreserved immediately after purification
and delivered frozen. Each vial contains >5 x 105 cells in 1
ml volume. HCPEC are characterized by immunofluorescent method with
antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of
DiI-Ac-LDL. HCPEC are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HCPEC are guaranteed to further expand
for 15 population doublings at the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No.
1001) for the culturing of HCPEC in vitro.
Product Use
HCPEC are for research use only. It is not approved for human or
animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen
upon receiving and keep the cells in liquid nitrogen until cell
culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Zheng, W. and Zhao, Q. (2004) Establishment and characterization
of an immortalized Z310 choroidal epithelial cell line from murine
choroids plexus. Brain Res. 958:371-380.
[2] Thomas, S. A., Bye, A. and Segal, M. B. (2001) Transport Characteristics
of the Anti-human Immunodeficiency Virus Nucleoside Analog, Abacavir,
into Brain and Cerebrospinal Fluid. J. Pharmacol. Exp. Ther. 298(3):947-53.
[3] Cornford, E. M., Hyman, S., Cornford, M. E. and Damian, R. T.
(1998) Glut1 glucose transporter in the primate choroids plexus
endothelium. J. Neuropathol. Exp. Neurol. 57:404-411.
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