Cell Specification
The choroid plexus epithelial cells (CPEpiC) are the structural basis, form a selective barrier between the nervous system and the blood, and secrete cerebrospinal fluid into the ventricles of the brain [1]. CPEpiC express inward-rectifying anion channels which are thought to have an important role in the secretion of cerebrospinal fluid [2]. CPEpiC synthesize and secrete many growth factors and other peptides that are of trophic benefit following injury to regions of the cerebroventricular system. For example, several growth factors are upregulated in choroid plexus after ischemic and traumatic insults to the central nervous system. The presence of numerous types of growth factor receptors in choroid plexus allows growth factor mediation of recovery processes by autocrine and paracrine mechanisms [3]. CPEpiC culture has been widely used to evaluate their morphological and physiological properties, and its capacity to serve as an in vitro model to access the cellular mechanisms of organic cation transport across the cerebrospinal fluid-blood barrier.

HCPEpiC from ScienCell Research Laboratories are isolated from human choroid plexus. HCPEpiC are cryopreserved immediately after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HCPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPEpiC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. No. 4101) for the culturing of HCPEpiC in vitro.

Product Use
HCPEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1]. Segal, M. B. (1993). Extracellular and cerebrospinal fluid. Journal of Inherited and Metabolic Disorder 16, 617-638.
[2]. Speake, T., Kajita, H., Smith, C. P. and Brown, P. D. (2004) Inward-rectifying anion channels are expressed in the epithelial cells of choroid plexus isolated from ClC-2 'knock-out' mice. J Physiol 539(Pt 2):385-90.
[3] Johanson CE, Palm DE, Primiano MJ, McMillan PN, Chan P, Knuckey NW, Stopa EG. (2000) Choroid plexus recovery after transient forebrain ischemia: role of growth factors and other repair mechanisms. Cell Mol Neurobiol 20(2):197-216.