Cell Specification
Fibroblasts are mesenchymal cells, which are derived from the embryonic mesoderm. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [1]. Fibroblasts have been isolated from many tissues and there is good evidence that in different parts of the body, they are intrinsically different [2]. Fibroblasts exhibit a characteristic spindle morphology and have a finite life span of about 50 generations in culture. Fibroblasts have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.

HCPF from ScienCell Research Laboratories are isolated from human choroid pluxus. HCPF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCPF are characterized by immunofluorescent method with antibody to fibronectin. HCPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPF are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HCPF in vitro.

Product Use
HCPF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.