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Human Meningeal Cells
(HMC)
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Catalog Number: 1400
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Cell Specification
Meningeal cells surrounding the brain participate actively in
the normal development of the central nervous system. For example,
they play important roles in both stabilizing the extracellular
matrix of the pial surface and by organizing the radial glial
scaffold and the lamination of the cerebellar cortex [1]. Selective
pharmacological destruction of the meningeal cells during a critical
ontogenetic period leads to specific malformation of both the
cerbella cortex and dentate gyrus [1]. Grafts of meningeal cells,
which derived form meninges overlying the cerebral cortex, in
adult rat spinal cord lesion promote axonal regrowth [2]. In
vitro study show that meningeal cell chemotactically orient
the migration of immature neurons but not glial cells [3].
HMC from ScienCell Research Laboratories are isolated
from human leptomeningi. HMC are cryopreserved at primary cultures
and delivered frozen. Each vial contains >5 x 105 cells in
1 ml volume. HMC are characterized by immunofluorescent method
with antibodies to fibronectin and negative to GFAP, a-smooth
muscle actin and Thy 1.1. HMC are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HMC are guaranteed to further
expand for 15 population doublings in the conditions specified
by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301)
for the culturing of HMC in vitro.
Product Use
HMC are for research use only. It is not approved for human or
animal use, or for application in in vitro diagnostic procedures.
Storage
Transfer directly and immediately cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992)
Destruction of meningeal cells over the medial cerebral hemisphere
of newborn hamster prevents the formation of the infrapyramidal
blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen,
J. (1999) Grafts of meningeal fibroblasts in adult rat spinal
cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal
cells stimulate and direct the migration of cerebellar external
granule cells in vitro. J. Neurocytol. 27:395-409.
