Cell Specification
The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variabilty in size and shape, all neurons share common morphologic features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain is known to contain about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons [2].

HN from ScienCell Research Laboratories are isolated from human brain. HN are cryopreserved at secondary cultures and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HN are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin 3. HN are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HN are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. No. 1521) for the culture of human neurons in vitro.

Product Use
HN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.