Cell Specification
The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool [1]. The EGL appears to be segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons of the brain; about 101 billion in man [2]. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that is expected to result from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation [3]. Cultured CGC are widely used as a model system for studying neuronal apoptosis.

HCGC from ScienCell Research Laboratories are isolated from human cerebellum. HCGC are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HCGC are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. HCGC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCGC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. No. 1521) for the culture of human cerebellar granule cells in vitro.

Product Use
HCGC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Hatten, M. E. (1999) Central nervous system neuronal migration. Annu. Rev. Neurosci. 22, pp. 511-539.
[2] Andersen, B.B., Korbo, L. and Pakkenberg, B. (1992) A quantitative study of the human cerebellum with unbiased stereological techniques. J. Comp. Neurol., 326:549-560.
[3] Monti, B, Marri, L, Contestabile, A. (2004) NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development. Eur J Neurosci. 16:1490-8.