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Human Oligodendrocyte Precursor Cell-oligospheres (HOPC-os)
Catalog Number: 1610	Pricing       Order       Reference       Product Sheet       Certificate of Analysis

Cell Specification
The precursor cells for oligodendrocytes were first discovered in 1993 by Raff, Miller and Noble [1] and have been extensively studied. These precursor cells are referred to in literature as either oligodendrocyte-type-2 astrocyte progenitor cells or oligodendrocyte precursor cells (OPC). The developing and adult central nervous systems both contain OPC [2, 3]. Oligodendrocytes, the myelin-forming cells of the central nervous system, develop from OPC. In culture, OPC can be generated from neural progenitors or neural stem cells in the presence of basic fibroblast growth factor and they proliferate in presence to platelet-derived growth factor or factors produced by astrocytes [4] and differentiate into mature oligodendrocytes. Because of this, they have provided an exceptional population in which to study developmental transitions.

HOPC-os from ScienCell Research Laboratories are isolated from human brain tissue. They are further purified from the oligodendrocyte precursor cell culture and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HOPC-os are characterized by immunofluorescent method with antibodies to A2B5 and nestin. Growing in differentiation medium, HOPC-os can be attached and differentiate to mature oligodendrcoytes. HOPC-os are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOPC-os are guaranteed to further expand in the condition specified by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Oligosphere Medium (OsM, Cat. No. 1611) for expanding oligospheres in vitro and use Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM, Cat. No. 1631) for the differentiation of oligospheres.

Product Use
HOPC-os are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Raff, M. C., Miller, R. H. and Noble, M. (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on the culture medium. Nature 303:390-396.
[2] ffrench-Constant, C. and Raff. M. C. (1986) Proliferating bipotential glial progenitor cells in adult rat optic nerve. Nature 319:499-502.
[3] Wolswijk, G. and Noble, M. (1989) Identification of an adult-specific glial progenitor cell. Development 105:387-400.
[4] Noble, M., Murray, K., Stroobant, P., Waterfield, M. D. Riddle, P. (1988) Platelet-derived growth factor promotes division and motility and inhibits premature differentiation of the oligodendrocyte/type-2 astrocyte progenitor cells. Nature 333:560-562.