Home ->Human -> CNS Cell System ->1620

Human Oligodendrocytes (HO)
Catalog Number: 1620	Pricing       Order       Reference       Product Sheet       Certificate of Analysis

Cell Specification
Oligodendrocytes, the myelin-forming cells of the central nervous system, are postmitotic cells that develop from oligodendrocyte precursor cells [1]. Oligodendrocytes, through their ability to myelinate axons, play an essential role in the mature nervous system [2]. Although the function of oligodendrocytes is well understood in rodent nervous system, little is known about the human origin of this cell type. The study of human oligodendrocyte biology and the treatment of white matter diseases would be facilitated by the ability to maintain cultures of enriched human oligodendrocytes or their progenitors [3].

HO from ScienCell Research Laboratories are derived from the differentiation of HOPC which isolated from human brain tissue. HO are cryopreserved immediately after purification from HOPC cultures and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HO are characterized by immunofluorescent method with antibodies to GalC and CNPase. HO are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HO are guaranteed to further culture in the conditions specified by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Oligodendrocyte Medium (OM, Cat. No. 1621) for the culturing of human oligodendrocyte in vitro.

Product Use
HO are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Zhang, S.-C., Ge, B. and Duncan, I. D. (2000) Tracing human oligodendroglial development in vitro. J. Neurosci. Res. 59:421-429.
[2] Woodruff, R. H. and Franklin, R. J. M. (1997) Growth factors and remyelination in the CNS. Histol. Histopathol. 12:459-466.
[3] Grever, W. E., Zhang, S.-C., Ge, B. and Duncan, I. D. (1999) Fractionation and enrichment of oligodendrocytes from developing human brain. J. Neurosci. Res. 57:304-314.