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Human Astrocytes-cerebellar
(HAc)
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Catalog Number: 1810
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Cell Specification
Astrocytes make up the majority of the cells in the mammalian
brain. They are the most variable in type, most intimately associated
with all parts of neurons, and thus most functionally interesting
in their relationships with neurons [1]. They provide structural,
trophic, and metabolic support to neurons and modulate synaptic
activity. Impairment of these astrocyte functions during stroke
and other insults can critically influence neuron survival. Furthermore,
astrocytes have been implicated in the pathological processes
of many neurological diseases [2]. Long-term recovery after brain
injury, through neurite outgrowth, synaptic plasticity, or neuron
regeneration, is influenced by astrocyte surface molecule expression
and trophic factor release [3]. In addition, the death or survival
of astrocytes themselves may affect the ultimate clinical outcome.
Recognition of the importance of astrocytes in nervous system
functioning is increasing, specifically regarding the modulation
of neural activity. Much of what we have learned about astrocytes
is from the in vitro studies and astrocyte cultures are
continuing to provide a useful tool in exploring the diverse property
of these cells.
HAc from ScienCell Research Laboratories are isolated
from human cerebellar tissue. HAc are cryopreserved at primary
culture and delivered frozen. Each vial contains >1 x 106
cells in 1 ml volume. HAc are characterized by immunofluorescent
method with antibody to GFAP. HAc are negative for HIV-1, HBV,
HCV, mycoplasma, bacteria, yeast and fungi. HAc are guaranteed
to further expand for 10 population doublings in the conditions
provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Astrocyte Medium (AM, Cat. No. 1801) for the culturing of human astrocytes in vitro.
Product Use
HAc are for research use only. They are not approved for human
or animal use, or for application in in vitro diagnostic
procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] G. I. Hatton (2004) Glial-neuronal interactions in the mammalian
brain. Adv. in Physiol. Edu. 26:225-237.
[2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J.
and Dijkstran, C. D. (1997) Extracellular matrix proteins expressed
by human adult astrocytes in vivo and in vitro: an astrocyte surface
protein containing the CS1 domain contributes to binding of lymphoblasts.
J. Neurosci. Res. 50:539-548.
[3] Chen Y., and Swanson, R. A. (2004) Astrocytes and brain injury.
J. Cereb. Blood Flow Metab. 23:137-149.
