Cell Specification
The dermal papilla is a highly active group of cells. It is derived from the dermis mesenchyme, located at the base of the hair follicle. The dermal papilla is implicated in controlling the hair growth cycle, be capable of inducing follicle development from the epidermis and production of hair fiber. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost [1]. Hair growth is tightly regulated by the epithelial-mesenchymal interaction of hair follicle cells, for example, interferon beta secreted from dermal papilla cells inhibits the growth of outer root sheath cells in cultured [2]. The survival of dermal papilla cell is also regulated by signal transduction pathways, activating both ERK and Akt promote dermal papilla cell survival [3].

HHDPC from ScienCell Research Laboratories are isolated from dermal papilla of human hair follicles. HHDPC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HHDPC are characterized by their mesenchymal cell morphology and immunofluorescent method with antibody to fibronectin and CD105. HHDPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHDPC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of HHDPC in vitro.

Product Use
HHDPC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Filsell, W., Little, J.C., Stones, A.J., Granger, S.P. and Bayley, S.A. (1994) Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype. Journal of Cell Science 107:1761-1772.
[2] Kim, C.D., Choe, Y., Shim, C., Kim, K. (2002) Interferon Beta secreted from human hair dermal papilla cells inhibits the growth of outer root sheath cells cultured in vitro. Biochem Biophys Res Commun. 290:1133-8.
[3] Han, J.H., Kwon, O.S., Chung, J.H., Cho, K.H., Eun, H.C., Kim, K.H. (2004) Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 34:91-8.