Cell Specification
The Germinal matrix is an area of reproducing cells situated around the papilla at the base of the hair bulb. It is the source of hair growth and transfer melanin to hair to give it pigmentation. During the active hair growth phase, cells in the matrix rapidly grow and differentiate, causing the hair to elongate. Cells of the germinal matrix under go a process called keratinization, which is the formation of a layer of the protein keratin, keeping the hair from falling out. Hair follicle has a complex immunologic profile, with immunologically "privileged" matrix cells [1]. Follicular cell implantation studies indicate that germinal matrix cells may induce new hair follicle formation by implanted dermal papilla cells [2].

HHGMC from ScienCell Research Laboratories are isolated from germinal matrix of hair follicle bulb. HHGMC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HHGMC are characterized by their mesenchymal cell morphology and immunofluorescent method with antibody to fibronectin and CD105. HHGMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHGMC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of HHGMC in vitro.

Product Use
HHGMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Pus, R., Cotsarelis, G. (2005) The biology of hair follicles. Mechanisms of Disease 341:491-497.
[2] Cooley, J. (2004) Follicular cell implantation: an update on "hair follicle cloning". Facial Plast Surg Clin N Am 12:219-224.