Home ->Human -> Lymphatic Cell System->2530

 

Human Lymphatic Fibroblasts (HLF)
Catalog Number: 2530	Pricing       Order       Reference       Product Sheet       Certificate of Analysis

Cell Specification
Fibroblasts are mesenchymal cells which are derived from the embryonic mesoderm. They have been used extensively for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli [3]. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.

HLF from ScienCell Research Laboratories are isolated from human lymph nodes. HLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLF are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HLF in vitro.

Product Use
HLF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2004) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 2004 Aug 30;450(3):291-6.