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Human Gingival Fibroblasts
(HGF)
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Catalog Number: 2620
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Cell Specification
Fibroblasts are mesenchymal cells with many vital functions
during development and in adult organisms. They are responsible
for much of the synthesis of extracellular matrix in connective
tissues and play major roles in wound healing. Many diseases
are associated with fibroblasts, either because fibroblasts
are implicated in their etiology or because of the fibrosis
that accompanies damage to other cell types in tissues. Fibroblasts
are among the most accessible normal mammalian cell types
and still the most amenable to culture in vitro. Gingival
fibroblasts are the major constituents of gingival tissue
and play a key role in their health maintenance [1]. Human
gingival fibroblasts (HGF) express a wide surface molecular
panel including mainly CD9, CD26, CD55, CD59, CD63, CD71,
CD86 CD95, CD99 and CD117 [2]. They were also demonstrated
to express mRNAs for protease-activated receptor-1 (PAR-1)
and PAR-3 [3].
HGF from ScienCell Research Laboratories are
isolated from human gingiva. HGF are cryopreserved at passage
one and delivered frozen. Each vial contains >5 x 105
cells in 1 ml volume. HGF are characterized by their spindle
morphology and immunofluorescent method with antibody to fibronectin.
HGF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,
yeast and fungi. HGF are guaranteed to further expand for
15 population doublings at the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No.2301) for the culturing of HGF in vitro.
Product Use
HGF are for research use only. It is not approved for human
or animal use, or for application in in vitro diagnostic
procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Poggi, P., Rodriguez y Baena, R., Rizzo, S., Rota, M.
T. (2004) Mouthrinses with alcohol: cytotoxic effects on human
gingival fibroblasts in vitro. J Periodontol. 74(5):623-9.
[2] Di Domenico, G., Del Vecchio, L., Postiglione, L., Ramaglia,
L. (2004) Immunophenotypic analysis of human gingival fibroblasts
and its regulation by Granulocyte-Macrophage Colony-Stimulating
Factor (GM-CSF). Minerva Stomatol. 52(3):81-7, 87-91.
[3] Tanaka, N., Morita, T., Nezu, A., Tanimura, A., Mizoguchi,
I., Tojyo, Y. (2004) Thrombin-induced Ca2+ mobilization in
human gingival fibroblasts is mediated by protease-activated
receptor-1 (PAR-1). Life Sci. 73(3):301-10.
