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Human Periodontal Ligament Fibroblasts
(HPLF)
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| Catalog Number: 2630 |
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Cell Specification
The periodontal ligament is a connective tissue located between
the cementum of teeth and the alveolar bone of the mandibula.
It plays an integral role in the maintenance and regeneration
of periodontal tissue. The cells responsible for maintaining this
tissue are thought to be fibroblasts, which can be either multipotent
or composed of heterogenous cell populations that differ in their
function. Despite the similar morphology with gingival fibroblasts,
periodontal ligament fibroblasts (PLF) appear to display distinct
functional activities in the maintenance of tissue integrity [1].
It is known that PLF produce osteoblast-related extracellular
matrix proteins and show higher alkaline phosphatase activity
compared with gingival fibroblasts [2]. PLF actively participate
in immune and inflammatory events in periodontal diseases [3].
HPLF from ScienCell Research Laboratories are isolated from human
periodontal tissue. HPLF are cryopreserved at passage one and
delivered frozen. Each vial contains >5 x 105 cells
in 1 ml volume. HPLF are characterized by their spindle morphology
and immunofluorescent method with antibody to fibronectin. HPLF
are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast
and fungi. HPLF are guaranteed to further expand for 15 population
doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HPLF in vitro.
Product Use
HPLF are for research use only. It is not approved for human or
animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Han, X., Amar, S. (2004) Identification of genes differentially
expressed in cultured human periodontal ligament fibroblasts vs.
human gingival fibroblasts by DNA microarray analysis. J Dent
Res. 81(6):399-405.
[2] Murakami, Y., Kojima, T., Nagasawa, T., Kobayashi, H., Ishikawa,
I. (2004) Novel isolation of alkaline phosphatase-positive subpopulation
from periodontal ligament fibroblasts. J Periodontol. 74(6):780-6.
[3] Takashiba, S., Naruishi, K., Murayama, Y. (2004) Perspective
of cytokine regulation for periodontal treatment: fibroblast biology.
J Periodontol. 74(1):103-10.
