|
Human Pulmonary Artery Endothelial Cells
(HPAEC)
|
| Catalog Number: 3100 |
|
Cell Specification
The vascular endothelial cells contribute to the maintenance of
vascular homeostasis. They synthesize and secrete activators as
well as inhibitors of both the coagulation system and the fibrinolysis
system, and mediators that influence the adhesion and aggregation
of blood platelets. Endothelial cells also release molecules that
control cell proliferation and modulate vessel wall tone. Activation
of adenosine receptors on human pulmonary artery endothelial cell
by lipopolysacchrides induces the release of IL-6 which may contribute
to the pathophysiology of acute lung injury [1]. Many of these,
and other, processes can be studied in vitro using cultured
cells and human pulmonary artery derived endothelial cells are
a commonly used cell type for such studies.
HPAEC from ScienCell Research Laboratories are isolated from
human pulmonary artery. HPAEC are cryopreserved at the end of
secondary culture and delivered frozen. Each vial contains >5
x 105 cells in 1 ml volume. HPAEC are characterized by immunofluorescent
method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and
by uptake of DiI-Ac-LDL. HPAEC are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HPAEC are guaranteed to
further expand for 15 population doublings at the conditions provided
by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No.
1001) for the culturing of HPAEC in vitro.
Product Use
HPAEC are for research use only. It is not approved for human
or animal use, or for application in in vitro diagnostic
procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Wilson, C. N., Batra, V. K. (2004) Lipopolysaccharide binds
to and activates A(1) adenosine receptors on human pulmonary artery
endothelial cells. J. Endotoxin Res. 8(4):263-71.
