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Human Pulmonary Artery Smooth Muscle
Cells
(HPASMC)
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| Catalog Number: 3110 |
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Cell Specification
Smooth muscle cell (SMC) is the cellular substate of most significant
arterial disease [1]. The increased growth potential of vascular
SMC represents one of the crucial anomalies responsible for the
development of essential vascular diseases. New studies demonstrate
that SMC express calcium channels [2] and the expression of ICAM-1
and VCAM-1 on SMC may contribute to the inflammatory reaction
in the vascular wall and may actively be involved in the progression
and stability of vascular disease [3]. Chronic lung hypoxia causes
vascular remodeling with pulmonary artery SMC hyperplasia, resulting
in pulmonary hypertension [4]. In vitro culture of human vascular
SMC as a model of vascular research played a critical role and
continues providing information in the pharmacology and the therapy
of vascular diseases.
HPASMC from ScienCell Research Laboratories are isolated from
human pulmonary artery. HPASMC are cryopreserved at secondary
culture after purification and delivered frozen. Each vial contains
>5 x 105 cells in 1 ml volume. HPASMC are characterized by
immunofluorescent method with antibodies to alpha-smooth muscle
actin and desmin. HPASMC are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HPASMC are guaranteed to further expand
for 15 population doublings at the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat.
No. 1101) for the culturing of HPASMC in vitro.
Product Use
HPASMC are for research use only. It is not approved for human
or animal use, or for application in in vitro diagnostic
procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication
of smooth muscle cells in vascular disease. Circ. Res.
58:427-444.
[2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2004) A 27 bp cis-acting
sequence is essential for L-type calcium channel alpha(1C) subunit
expression in vascular smooth muscle cells. Biochim Biophys
Acta. 1577:401-11.
[3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S.
B. (1999) Cellular adhesion molecules on vascular smooth muscle
cells. Cardiovasc. Res. 41:395-401.
[4] Rose, F., et al. (2004) Hypoxic pulmonary artery fibroblasts
trigger proliferation of vascular smooth muscle cells: role of
hypoxia-inducible transcription factors. FASEB J. 12:1660-1.
