Cell Specification
Skeletal muscle cells are one of the largest cells in the body. They are multinucleate formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process in which many factors are involved. When skeletal muscle suffers an injury, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and finally differentiate [1]. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth [2]. Insulin-stimulated glucose transport in cultured human skeletal muscle is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation [3]. The fusion of mononucleatcd cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying this process of cell differentiation.

HSkMC from ScienCell Research Laboratories are isolated from human skeletal muscle of the pectoral girdle. HSkMC are cryopreserved on primary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HSkMC are characterized by immunofluorescent method with antibodies to myosin, actin and actinin. HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Skeletal Muscle Cell Medium (SkMCM, Cat. No. 3501) for the culturing of HSkMC in vitro.

Product Use
HSkMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1]. Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78.
[2]. Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404.
[3]. Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.