Cell Specification
The urinary bladder is a hollow organ made up of smooth muscle cells. The relaxation and contraction of urinary bladder smooth muscle allows the bladder to store and void urine, respectively. Phenotypic modulation of bladder smooth muscle cells and the expression of inducible nitric oxide synthase are associated with various pathological conditions, including bladder dysfunction [1]. Studies have shown that hypoxia inhibits human bladder smooth muscle cell proliferation [2] and bladder smooth muscle cell differentiation is dependent on the factor(s) released from urothelial cells [3]. The secretory phenotype of bladder smooth muscle cell ECM can be altered by the frequency of mechanical deformation experienced by the cells. Since smooth muscle is the final common pathway for many diseases, to understand how smooth muscle changes during the genesis and maintenance of a disease is an important step toward the development of therapeutic approaches.

HBdSMC from ScienCell Research Laboratories are isolated from human bladder tissue. HBdSMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBdSMC are characterized by immunofluorescent method with antibodies to -smooth muscle actin and desmin. HBdSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSMC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. No. 1101) for the culturing of HBdSMC in vitro.

Product Use
HBdSMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Johansson, R. and Persson, K. (2004) Phenotypic modulation of cultured bladder smooth muscle cells and the expression of inducible nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol 286(4):R642-8.
[2] Galvin, D. J., Watson, R. W., O'Neill, A., Coffey, R. N., Taylor, C., Gillespie, J. I., Fitzpatrick, J. M. (2004) Hypoxia inhibits human bladder smooth muscle cell proliferation: a potential mechanism of bladder dysfunction. Neurourol Urodyn 23(4):342-8.
[3] Liu, W., Li, Y., Hayward, S., Cunha, G. and Baskin, L. (2000) Diffusable growth factors induce bladder smooth muscle differentiation. In Vitro Cell Dev Biol Anim. 36(7):476-84.