Cell Specification
Human synoviocytes (HS), the predominant cell type of healthy synovial tissue, are fibroblast-like cells. The Synoviocytes form a distinct structure called the synovial lining layer. Electron microscopy revealed extensive cell-to-cell contacts within the lining layer [1]. The Synoviocytes produce synovial fluid components and are responsible for absorption from the joint cavity, and for blood/synovial fluid exchanges. The synoviocytes proliferate, show anchorage-independent growth, and also secrete a variety of effector molecules that promote inflammation and joint destruction and, themselves are part of a complex network of autocrine and paracrine acting factors. The synoviocytes express cadherin-11 providing new insight into synovial tissue organization and morphogenesis [2] and of interest as a therapeutic target.

HS from ScienCell Research Laboratories are isolated from human synovium. HS are cryopreserved at passage one cultures and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HS are characterized by their fibroblast-like morphology, growth pattern and immunocytochemistry of CD 90 and fibronectin. HS are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HS are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Synoviocyte Medium (SM, Cat. No. 4701) for the culturing of human synoviocytes in vitro.

Product Use
HS are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1]. Barland, P., Novikoff, A.B., Hamerman, D. (1962) Electron microscopy of the human synovial membrane. J Cell Biol. 14:207-220.
[2]. Kiener , H. P. and Brenner, M. B. (2005) Building the synovium: cadherin-11 mediates fibroblast-like synoviocyte cell-to-cell adhesion. Arthritis Res Ther. 7(2): 49-54.