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Human Hepatocytes
(HH)
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| Catalog Number: 5200 |
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Cell Specification
The liver fulfills many vital processes in mammals. It is the
central organ of energy metabolism, biotransformation of xenobiotics,
and synthesis of plasma proteins under physiological and pathophysiological
conditions [1]. Primary culture of human hepatocytes appears to
be a suitable experimental model for the study of liver specific
function, have been and still are an important tool. Propagation
of human hepatocytes for cell transplantation, gene therapy, and
culture of hepatocytes in bioartificial liver support systems
is now under investigation [2]. In appropriate culture condition,
cultured human hepatocytes proliferate and maintain differentiated
hepatocyte function such as the synthesis of serum proteins [3].
HH from ScienCell Research Laboratories are isolated from human
liver tissue. HH are cryopreserved at secondary culture after
purification and delivered frozen. Each vial contains >1 x
106 cells in 1 ml volume. HH are characterized by immunofluorescent
method with antibodies to albumin, cytokeratin-18 and vimentin.
HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast
and fungi. HH are guaranteed to further culture in the conditions
provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Hepatocyte Medium (HM, Cat. No.
5201) for the culturing of human hepatocytes in vitro.
Product Use
HH are for research use only. It is not approved for human or
animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Runge, D., Michalopoulos, G. K., Strom, S. C. and Runge, D.
M. (2000) Recent advances in human hepatocyte culture systems.
Biochem. Biophysi. Res. Comm. 274:1-3.
[2] Chen, H. L., Wu, H. L., Fon, C. C., Chen, P. J., Lai, M. Y.
and Chen, D. S. (1998) Long-term culture of hepatocytes from human
adults. J. Biomed. Sci. 5:435-440.
[3] Okamoto, M., Ishida, Y., Keogh, A. and Strain, A. (1998) Evaluation
of the function of primary human hepatocytes co-cultured with
the human hepatic stellate cell (HSC) line LI90. Int. J. Artif.
Organs 21:353-359.
