Cell Specification
Smooth muscle cell (SMC) is the cellular substate of most significant arterial disease [1]. The increased growth potential of vascular SMC represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that SMC express calcium channels [2] and the expression of ICAM-1 and VCAM-1 on SMC may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease [3]. In vitro culture of human vascular SMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.

HASMC from ScienCell Research Laboratories are isolated from human aorta. HASMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HASMC are characterized by immunofluorescent method with antibodies to alpha-smooth muscle actin and desmin. HASMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HASMC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. No. 1101) for the culturing of HASMC in vitro.

Product Use
HASMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444.
[2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2004) A 27 bp cis-acting sequence is essential for L-type calcium channel alpha(1C) subunit expression in vascular smooth muscle cells. Biochim Biophys Acta. 1577:401-11.
[3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401.