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Human Cardiac Fibroblasts-adult atrial
(HCFaa)
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| Catalog Number: 6320 |
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Cell Specification
The cardiac fibroblasts (CF) are the principal cell type of the
heart. They provide structural support for cardiac myocytes and
are responsible for extracellular matrix synthesis in the heart
during growth and pathophysiological conditions. CF are an important
cellular component of myocardial responses to injury and the source
of paracrine growth factors. Their proliferation and synthesis
of matrix is essential for scar formation at sites of myocardial
infarction [1], cardiac fibrosis, and is often complicated by
cardiac hypertrophy [2]. CF also respond to changing environments
in pathophysiological conditions by modulating integrin expression
[3]. CF culture has been widely used as a model to study the cardiac
matrix remodeled by physiological (exercise) and pathological
(hypertension) stressors.
HCF from ScienCell Research Laboratories are isolated from human
heart. HCF are cryopreserved at secondary culture and delivered
frozen. Each vial contains >5 x 105 cells in 1 ml volume.
HCF are characterized by immunofluorescent method with antibody
to fibronectin. HCF are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HCF are guaranteed to further expand
for 15 population doublings at the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HCF in vitro.
Product Use
HCF are for research use only. It is not approved for human or
animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2004) Protease-activated
receptor-1-mediated DNA synthesis in cardiac fibroblast is via
epidermal growth factor receptor transactivation: distinct PAR-1
signaling pathways in cardiac fibroblasts and cardiomyocytes.
Circ. Res. 91:532-9.
[2] Akiyama-Uchida, Y. et al., (2004) Norepinephrine enhances
fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension
40:148-54.
[3] Burgess ML, Terracio L, Hirozane T, Borg TK. Differential
integrin expression by cardiac fibroblasts from hypertensive and
exercise-trained rat hearts. Cardiovasc Pathol 2004 Mar-Apr;11(2):78-87.
