Cell Specification
The mammary gland consists of pseudostratified epithelia, with myoepithelila cells resting upon a basement membrane and apical glandular epithelial cells facing the lumen of the ducts and alveoli. Mammary epithelial cells undergo changes in growth, invasion, and differentiation throughout much of adulthood, and most strikingly during pregnancy, lactation, and involution. They proliferate and invade the surrounding extracellular matrix during the fetal and postnatal development of the gland, and then more vigorously at puberty as the branches of the mammary epithelial tree are formed [1, 2].

HMEpiC from ScienCell Research Laboratories are isolated from human breast tissue. HMEpiC are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HMEpiC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HMEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMEpiC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Mammary Epithelial Cell Medium-2 (MEpiCM2, Cat. No. 7611) for the culturing of HMEpiCin vitro.

Product Use
HMEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1]. Topper, Y.J., Freeman, C.S. (1980) Multiple hormone interactions in the developmental biology of the mammary gland. Physiol Rev 60:1049-106.
[2]. Hennighausen, L., Robinson, G.W. (2001) Signaling pathways in mammary gland development. Dev Cell 1:467-475.