Cell Specification
Fibroblasts are the most common types of mammary stromal cells. Mammary stromal cells differentiate into fibroblasts, adipocytes or vascular structures in a hormone- and substatum-dependent manner, and may explain the dramatic changes in stromal composition during both normal mammary gland development and tumorigenesis [1]. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. The Mammary stromal fibroblasts express and produce cytokines such as SF witch plays roles in mammary cancer [2].

HMF from ScienCell Research Laboratories are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HMF in vitro.

Product Use
HMF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1]. Topper, Y.J., Freeman, C.S. (1980) Multiple hormone interactions in the developmental biology of the mammary gland. Physiol Rev 60:1049-106.
[2]. Hennighausen, L., Robinson, G.W. (2001) Signaling pathways in mammary gland development. Dev Cell 1:467-475.