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Human Umbilical Artery Endothelial Cells
(HUAEC)
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| Catalog Number: 8010 |
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Cell Specification
The vascular endothelial cells contribute to the maintenance of
vascular homeostasis. They synthesize and secrete activators as
well as inhibitors of both the coagulation system and the fibrinolysis
system, and mediators that influence the adhesion and aggregation
of blood platelets. Endothelial cells also release molecules that
control cell proliferation and modulate vessel wall tone. Many
of these, and other, processes can be studied in vitro using cultured
cells, and human umbilical vein and artery endothelial cells are
the most commonly used cell types for such studies. Except for
the common endothelial cell features such as "cobblestone" morphology,
positive staining for Factors VIII and the ability to take up
acetylated low-density lipoprotein [1], human umbilical endothelial
cells also stein positively with CD-31 [2]. Cells pretreated with
IL-1 or TNF-a also selectively express E-selectin [3] and VCAM
[4].
HUAEC from ScienCell Research Laboratories are isolated from
human umbilical artery. HUAEC are cryopreserved at the end of
secondary culture and delivered frozen. Each vial contains >5
x 105 cells in 1 ml volume. HUAEC are characterized by immunofluorescent
method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and
by uptake of DiI-Ac-LDL. HUAEC are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HUAEC are guaranteed to
further expand for 15 population doublings at the conditions provided
by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No.
1001) for the culturing of HUAEC in
vitro.
Product Use
HUAEC are for research use only. It is not approved for human
or animal use, or for application in in vitro diagnostic
procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid
nitrogen upon receiving and keep the cells in liquid nitrogen
until cell culture needed for experiments.
Shipping
Dry ice.
Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical
vein endothelial cells. In "Human Cell Culture Protocols" (Gareth
E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation
to adhesion molecules of the immunoglobulin gene superfamily Science
247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion
molecule 1: an inducible receptor for neutrophils related to complement
regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion
molecule 1, a cytokine induced endothelial protein that binds
to lymphocytes. Cell 59:1203-1211.
