Cell Specification
Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle [1, 2]. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs.

DMSC-ad from ScienCell Research Laboratories are isolated from dog adipose tissue. DMSC-ad are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. DMSC-ad are characterized by immunofluorescent method with antibodies to CD44, CD90 and lipid staining after differentiation. DMSC-ad are negative for mycoplasma, bacteria, yeast and fungi. DMSC-ad are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of DMSC-ad in vitro.

Product Use
DMSC-ad are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74.
2. Barry, F. P., and J. M. Murphy. Mesenchymal stem cells: clinical applications and biological characterization. 2004. Int J Biochem Cell Biol. 36(4):568-84.