During the past two decades, researchers have been seeking
serum-free media for the culturing of a wide range of cell lines
and even primary cells. With the supplement of growth factors
and hormones, researcher found the medium to be most satisfactory
for study of variety of cell types was a 1:1 mixture of DMEM
and Ham's F-12 Nutrient Mixture. HEPES buffer is included in
the formulation at a final concentration of 15 mM to compensate
for the loss of buffering capacity incurred by eliminating serum.
Human Umbilical Mesenchymal
Stem Cells: The human umbilical mesenchymal stem cells
just been added to our mesenchymal stem cell inventory and
is available for your purchase. These cells were isolated
from Wharton's Jelly of umbilical cord and cryopreserved
on primary culture. MSC can be induced to transform into
neurons, glia, cardiomyocytes, adipogenic and osteogenic
lineages.
