Cell Specification
The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variabilty in size and shape, all neurons share common morphologic features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system.

The MN-r from ScienCell Research Laboratories are isolated from E14 mouse brain raphe. MN-r are cryopreserved at primary cultures and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. MN-r are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. MN-r are negative for mycoplasma, bacteria, yeast and fungi. MN-r are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. No. 1521) for the culture of mouse neurons in vitro.

Product Use
MN-r are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.