Cell Specification
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases [3].

MLF from ScienCell Research Laboratories are isolated from mouse lymph nodes. MLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. MLF are negative mycoplasma, bacteria, yeast and fungi. MLF are guaranteed to further expand for 10 population doublings at the condition provided by ScienCell Research Laboratories.

Product Use
MLF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob'ev, A. L. (1984) Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.