proteins

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Home -> Proteins

Normal Human Primary Cell Lysate

Preparation: Cell lysate was prepared in modified RIPA buffer (50mMTris, pH7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40 , 1mM Na₃VO₄, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted and boiled for 5 min with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol.

Concentration: 2 mg/ml

Storage: Stable for 6 months at -20^oC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Application: Cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Cat. No.	Product Name	$340	$470
1106	Human brain vascular smooth muscle cell lysate	200 µg	400 µg
1206	Human brain vascular pericyte lysate	200 µg	400 µg
1406	Human meningeal cell lysate	200 µg	400 µg
1526	Human neuron lysate	200 µg	400 µg
1606	Human oligodendrocyte precursor cell lysate	200 µg	400 µg
1716	Human perineurial cell lysate	200 µg	400 µg
1806	Human astrocyte lysate	200 µg	400 µg
1906	Human microglia lysate	200 µg	400 µg
2006	Human dermal microvascular endothelial cell lysate	200 µg	400 µg
2016	Human dermal lymphatic endothelial cell lysate	200 µg	400 µg
2106	Human dermal kerotinocyte lysate	200 µg	400 µg
2206	Human dermal melanocyte lysate	200 µg	400 µg
2306	Human dermal fibroblast lysate	200 µg	400 µg
2506	Human lymphatic endothelial cell lysate	200 µg	400 µg
2536	Human lymphatic fibroblast lysate	200 µg	400 µg
2626	Human gingival fibroblast lysate	200 µg	400 µg
2636	Human peridontal ligmant firbroblast lysate	200 µg	400 µg
2716	Human esophageal smooth muscle cell lysate	200 µg	400 µg
2906	Human intestinal microvascular endothelial cell lysate	200 µg	400 µg
2916	Human intestinal smooth muscle cell lysate	200 µg	400 µg
3006	Human pulmonary microvascular endothelial cell lysate	200 µg	400 µg
3106	Human pumonary artery endothelial cell lysate	200 µg	400 µg
3116	Human pulmonary artery smooth muscle cell lysate	200 µg	400 µg
3126	Human pulmonary artery fibroblast lysate	200 µg	400 µg
3206	Human pulmonary alveolar epithelial cell lysate	200 µg	400 µg
3306	Human pulmonary fibroblast lysate	200 µg	400 µg
3406	Human bronchial smooth muscle cell lysate	200 µg	400 µg
3416	Human tracheal smooth muscle cell lysate	200 µg	400 µg
3506	Human skeletal muscle cell lysate	200 µg	400 µg
4006	Human renal glomerular endothelial cell lysate	200 µg	400 µg
4106	Human renal proximal tubular epithelial cell lysate	200 µg	400 µg
4116	Human renal cortical epithelial cell lysate	200 µg	400 µg
4126	Human renal epithelial cell lysate	200 µg	400 µg
4206	Human renal mesangial cell lysate	200 µg	400 µg
4306	Human bladder microvascular endothelial cell lysate	200 µg	400 µg
4316	Human bladder smooth muscle cell lysate	200 µg	400 µg
4336	Human bladder stromal fibroblast lysate	200 µg	400 µg
4606	Human calvarial osteoblast lysate	200 µg	400 µg
5006	Human hepatic sinusoidal endothelial cell lysate	200 µg	400 µg
5106	Human intraheptic biliary epithelial cell lysate	200 µg	400 µg
5206	Human hepatocyte lysate	200 µg	400 µg
6006	Human cardiac microvascular endothelial cell lysate	200 µg	400 µg
6106	Human aortic endothelial cell lysate	200 µg	400 µg
6116	Human aortic smooth muscle cell lysate	200 µg	400 µg
6206	Human cardiac myocyte lysate	200 µg	400 µg
6526	Human keratocyte lysate	200 µg	400 µg
6546	Human retinal pigment epithelial cell lysate	200 µg	400 µg
6556	Human lens epithelial cell lysate	200 µg	400 µg
6566	Human iris pigment epithelial cell lysate	200 µg	400 µg
6586	Human non-pigment ciliary epithelial cell lysate	200 µg	400 µg
6596	Human trabecular meshwork cell lysate	200 µg	400 µg
7106	Human amniotic epithelial cell lysate	200 µg	400 µg
7126	Human villous trophoblast lysate	200 µg	400 µg
7136	Human villous mesenchymal fibroblast lysate	200 µg	400 µg
7216	Human preadipocyte lysate	200 µg	400 µg
7616	Human mammary epithelial cell lysate	200 µg	400 µg
7636	Human mammary fibroblast lysate	200 µg	400 µg
8006	Human umbilical vein endothelial cell lysate	200 µg	400 µg
8016	Human umbilical artery endothelial cell lysate	200 µg	400 µg
8026	Human umbilical vein smooth muscle cell lysate	200 µg	400 µg
8036	Human umbilical artery smooth muscle cell lysate	200 µg	400 µg

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