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Normal Human Primary Cell cDNA

ScienCell Research Laboratories' PCR-ready first strand cDNA is an excellent source of cell specific, PCR-ready, first strand cDNA. It can be used immediately for gene discovery or expression analysis. ScienCell Research Laboratories provides the largest selection of normal human cell cDNAs for academic and biomedical industry research.

The first-strand cDNA is synthesized from RNA isolated from a wide variety of normal human cell types. Total RNA used for cDNA synthesis is isolated by Qiagen RNeasy kit. Ten micrograms of total RNA is primed with random primer oligonucleotides and reverse-transcribed by MMLV reverse transcriptase in 40 µl final volume. RT reaction is stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer. (1x RT Buffer: 50 mM Tris-HCl, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5' end of human beta-actin cDNA has been amplified by PCR from all cDNAs.

Features

  • Full-length first strand cDNA synthesis.
  • Ready for PCR amplification.
  • Templates for second strand synthesis and construction of cDNA libraries.
  • The largest selection of cDNA from different normal human cell types.

Applications

  • Immediate PCR amplification of known genes .
  • Comparison of gene expression patterns between biological samples .
  • Gene mutation analysis .
  • Analysis of mRNA alternative splicing .
  • Gene cloning and target sequencing .

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ScienCell’s genomic DNA is isolated from early passage of over 100 different human primary cell types by Qiagen Allprep kit. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0. Our genomic DNA is provided ready to use for SNP analysis, DNA methylation analysis, Southern Blotting, and PCR. Getting genomic DNA from ScienCell will save you valuable research time to immediately identify genes of interest; no longer will you need to acquire tissue or cells and isolate genomic DNA by yourself.

Features

  • Genomic DNA isolated from a wide variety of human primary cells .
  • Decontamination of polysaccharide and proteoglycan .
  • Extensive quality control procedures ensure high quality genomic DNA .
  • High efficiency in PCR .

Applications

  • PCR, SNP analysis and Southern Blotting .
  • Genomic DNA library construction.
  • Genomic DNA methylation study.
  • Profiling study in gene expression.

Click here to view all Genomic DNA