Molecular Biology

Molecular biology is the study of molecular process of replication, transcription and translation of the genetic material. Much of the work in molecular biology is quantitative, and recently much work has been done at the interface of molecular biology and computer science in bioinformatics and computational biology.

Subcategories:

DNADNA

RNARNA


ProteinsProteins

Normal Human Primary Cell Lysate

Preparation: Cell lysate was prepared in modified RIPA buffer (50mMTris, pH7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40 , 1mM Na3VO4, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted and boiled for 5 min with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol.

Concentration: 2 mg/ml

Storage: Stable for 6 months at -20oC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Application: Cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.