Mesenchymal Stem Cell medium-serum free-basal (MSCM-sf-b) is a basal medium designed for optimal growth and expansion of normal human mesenchymal stem cells (MSCs) in vitro in conjunction with mesenchymal stem cell growth supplement serum free (MSCGS-sf, Cat. No. 7562) and penicillin/streptomycin solution (P/S, Cat. No. 0503). MSCs can be expanded for several passages while maintaining their multipotential phenotype. MSCM-sf-b is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and trace minerals. The basal medium does not contain antimycotics, antibiotics, hormones or growth factors. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
MSCM-sf-b is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.