Mouse Macrophage
[MMa-bm]

Catalog Number: #M1920

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Description

Macrophages are cells produced by the differentiation of monocytes in tissues. Macrophages are large cells found within many types of tissue. They derive from monocytes that have entered tissues. Macrophages phagocytise invading microorganisms and also scavenge dead and damaged cells and cellular debris. In the bone marrow and subsequestly in the blood and tissues, cells of this series undergo a series of functional and morphologic maturation steps that culminate in the mature tissue macrophage [1]. Macrogphage can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3 and CD68 by flow cytometory or immunohistochemical staining [2].

MMa-bm from ScienCell Research Laboratories are isolated from adult mouse bone marrow. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MMa-bm is characterized by immunofluorescent method with antibody to CD 11b. MMa-bm is negative for mycoplasma, bacteria, yeast and fungi. MMa-bm is guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. No. 1921) for the culturing of MMa-bm in vitro.

Product Use

MMa-bm are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69.
[2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology 5(12) 953-964.