Human Tonsil Fibroblasts (HTF)
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been
extensively used for a wide range of cellular and molecular studies. This is mainly
because they are one of easiest types of cells to grow in culture, and their durability
makes them amenable to a wide variety of manipulations ranging from studies employing
gene transfection to microinjection. There is good evidence that fibroblasts in different
parts of the body are intrinsically different . Fibroblasts within tissues are exposed to a
dynamic mechnical environment, which influences the structure integrity of both healthy
and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in
type I and/or type III collagen  They are responsible for much of the synthesis of
extracellular matrix in connective tissues and play major roles in wound healing. Many
diseases are associated with fibroblasts, either because fibroblasts are implicated in their
etiology or because of the fibrosis that accompanies damage to other cell types in tissues.
Fibroblasts are among the most accessible normal mammalian cell types and still the
most amenable to culture in vitro.
HTF from ScienCell Research Laboratories are isolated from human tonsils. HTF are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml
volume. HTF are characterized by their spindle morphology and immunofluorescent method
with antibody to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,
yeast and fungi. HTF are guaranteed to further expand for 15 population doublings at the
condition provided by ScienCell Research Laboratories.
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HTF in
HTF are for research use only. It is not approved for human or animal use, or for application in
in vitro diagnostic procedures.
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture needed for experiments.
 Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea,
heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
 Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn,
ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.