L-Lactate Assay (LAC)
L-Lactate is an important intermediary in glucose metabolism. Under hypoxic or anaerobic conditions, lactate
dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, to lactate. Monitoring lactate levels is,
therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when
studying cellular and animal physiology. This colorimetric assay is based on the reduction of the terazolium salt
INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The
intensity of the absorbance is proportional to the lactate concentration.
L-Lactate Assay kit could measure lactate level of samples from cells, serum, plasma, cell culture media and
tissue extracts. This product is for research purposes only and not for use in animals, humans, or diagnostic
The kit would be shipped on dry ice and the kit is stable for half a year when handled properly.