Citrate Synthase Assay (CS)

Catalog #8318 

 
    

Description

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction between acetyl coenzyme A (acetyl CoA) and oxaloacetic acid to form citric acid and CoA with a thiol group (CoA-SH). This colorimetric assay is based on the reaction between 5, 5-Dithiobis 2-nitrobenzoic acid (DTNB) and CoA-SH to form TNB, which exhibits an absorbance maximum at 412 nm. The intensity of the absorbance is proportional to the citrate synthase activity. This enzyme is an exclusive marker of the mitochondrial matrix1.


Product Use

This kit is used for the fast and simple measurement of citrate synthase activity2 as well as the detection of intact mitochondrial inner membrane. It is for research purposes only and not for use in animals, humans, or diagnostic procedures.


Shipping

The kit would be shipped on dry ice


Reference

1. Morgunov I and Srere PA. Interacttion between citrate synthase and malate dehydrogenase substrate channeling of oxaloacetate. 1998. J. Biol. Chem 273: 29540-29544
2. Trounce IA, Kim YL, Jun AS, Wallace DC. Assessment of mitochondrial oxidative phosphorylation in patient muscle. biopsies, lymphoblasts, and transmitochondrial cell lines. 1996. Methods Enzymol 264: 484 509