Alanine Transaminase Assay (ALT)
Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), catalyzes the
reversible transfer of an amino group from alanine to α-ketoglutarate. The products of this transamination
reaction are pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as
well. Significantly elevated serum ALT levels often suggest the existence of medical problems, such as
hepatocellular injury, hepatitis, diabetes, bile duct problem and myopathy. This colorimetric assay is based on a
based on the oxidization of NADH to NAD in the presence of pyruvate and lactate dehydrogenase. The ALT
activity is determined by assaying the rate of NADH oxidation, which is proportional to the reduction in
absorbance at 340nm over time (ΔOD340nm/min).
The Alanine Transaminase Assay kit measures the alanine transaminase activity of different types of samples,
such as serum, plasma and tissues. This product is for research purposes only and not for use in animals,
humans, or diagnostic procedures.
Store in -20°C
Shipped on dry ice and stable for three months.