Description

Medium 199 with Hank's salts and L-glutamine, 500 ml Media 199 were formulated to promote continuous growth of primary chick embryo fibroblasts without serum. The medium is useful with serum, for growth of a broad range of cells. Media 199 are formulated with either Earle's salts or Hank's salts Media utilizing Hank's salts are buffered with salt solutions designed for atmospheric equilibration and use in a CO2 incubator will result in a rapid drop in pH of the culture medium.

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