Smooth muscle cell-serum free medium (SMCM-sf) is designed for optimal growth and expansion of normal human smooth muscle cells (SMCs) in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment at serum-free condition that selectively promotes growth of normal human smooth muscle cells in vitro.
Components
SMCM-sf consists of 500 ml of basal medium, 5 ml of smooth muscle cell growth supplement serum free (SMCGS-sf, Cat. No. 1162) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
SMCM-sf is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the SMCGS-sf and the P/S solution at -20oC. Protect from light.
