Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM) is a complete medium designed for the differentiation of oligodendrocyte precursor cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (1%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes the differentiation of normal human oligodendrocyte precursor cells in vitro.
Components
OPCDM consists of 500 ml of basal medium, 5 ml of fetal bovine serum (FBS, Cat. No. 0005), 5 ml of oligodendrocyte precursor cell differentiation supplement (OPCDS, Cat. No. 1672), and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
OPCDM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the OPCDS, the FBS and the P/S solution at -20°C. Protect from light.
