Melanocyte Medium (MelM) is a complete medium designed for optimal growth of normal human epidermal-derived melanocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (0.5%). It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human melanocytes in vitro.
Components
MelM consists of 500 ml of basal medium, 5 ml of melanocyte growth supplement (MelGS, Cat. No. 2252), 2.5 ml of fetal bovine serum (FBS, Cat. No. 0002) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
MelM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the MelGS, FBS and the P/S solution at -20°C. Protect from light.
