Melanocyte Medium-2 (MelM-2) is a TPA-free complete medium designed for optimal growth of normal human epidermal-derived melanocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (0.5%). It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human melanocytes in vitro.
Components
MelM-2 consists of 500 ml of basal medium, 5 ml of TPA-free melanocyte growth supplement (MelGS-2, Cat. No. 2262), 2.5 ml of fetal bovine serum (FBS, Cat. No. 0002) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
MelM-2 is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the MelGS, FBS and the P/S solution at -20°C. Protect from light.
