Oral Keratinocyte Medium (OKM) is a complete medium designed for optimal growth of normal human oral keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human oral keratinocytes in vitro.
Components
OKM consists of 500 ml of basal medium, 5 ml of oral keratinocyte growth supplement (OKGS, Cat. No. 2652) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
OKM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the OKGS and the P/S solution at -20°C. Protect from light.
