Epithelial Cell Medium (EpiCM) is a complete medium designed for optimal growth of normal human epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human epithelial cells in vitro.
Components
EpiCM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of epithelial cell growth supplement (EpiCGS, Cat. No. 4152) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
EpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the EpiCGS, the FBS and the P/S solution at -20°C. Protect from light.
