Description

Nucleus pulposus is the jelly-like substance in the middle of the intervertebral disc. It functions to distribute hydraulic pressure in all directions within each disc under compressive loads. The nucleus pulposus consists of chondrocyte-like cells, collagen fibrils, and proteoglycan aggrecans. Nucleus pulposus cells reside in an environment that has a limited vascular supply and generate energy through anaerobic glycolysis. The nucleus pulposus cells express hypoxia-inducible factor-1 [1], which responds to oxygen tension and regulates glycolysis. They express cytokeratin(s) and vimentin in fetal life and childhood; and secrete interleukins-1?, -6, and -10 and granulocyte-macrophage colony-stimulating factor in cell culture [2]. The nucleus pulposus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc diorders.

HNPC from ScienCell Research Laboratories are isolated from nucleus pulposus of human intervertebral discs. HNPC are cryopreserved at either primary or passage one culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HNPC are characterized by immunofluorescent method with cytokaratin and vimentin antibodies. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HNPC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Nucleus Pulposus Cell Medium (NPCM, Cat. No. 4801) for the culturing of human nucleus pulposus cells in vitro.

Product Use

HNPC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference