Nucleus Pulposus Cell Medium (NPCM) is a complete medium designed for optimal growth of normal human nucleus pulposus cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human nucleus pulposus cells in vitro.
Components
NPCM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of Nucleus Pulposus Cell Growth Supplement (NPCGS, Cat. No. 4852) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
NPCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the NPCGS, the FBS and the P/S solution at -20°C. Protect from light.
