Corneal Epithelial Cell Medium (CEpiCM) is a complete medium designed for optimal growth of normal human corneal epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human corneal epithelial cells in vitro.
Components
CEpiCM consists of 500 ml of basal medium, 5 ml of corneal epithelial cell growth supplement (CEpiCGS, Cat. No. 6552) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
CEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the CEpiCGS and the P/S solution at -20°C. Protect from light.
