Our adipogenesis differentiation medium has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis study. Generally, with this medium, we obtain >35% mature adipocytes from human bone marrow, >50% from rat bone marrow, and >60% from human mesenchymal stem cell isolated from adipose tissue. Efficiency of adipogenic differentiation depends on the quanlity of the mesenchymal stem cells.
Mesenchymal Stem Cell adipogenic differentiation medium (MADM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
Components
MADM(500ml) consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of mesenchymal stem cell adipogenic differentiation supplement (MADS-L, Cat. No. 7542-L), 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
MADM-L is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the MADS-L and the P/S solution at -20°C. Protect from light.
Shipping
Gel ice.
Mesenchymal Stem Cell Adipogenic Differentiation Medium
(MADM)
Our adipogenesis differentiation medium has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis study. Generally, with this medium, we obtain >35% mature adipocytes from human bone marrow, >50% from rat bone marrow, and >60% from human mesenchymal stem cell isolated from adipose tissue. Efficiency of adipogenic differentiation depends on the quanlity of the mesenchymal stem cells.
Mesenchymal Stem Cell adipogenic differentiation medium (MADM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
Components
MADM(250ml) consists of 250 ml of basal medium, 12.5 ml of fetal bovine serum (FBS, Cat. No. 0012), 2.5 ml of mesenchymal stem cell adipogenic differentiation supplement (MADS-S, Cat. No. 7542-S), 2.5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503-S).
Product Use
MADM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the MADS-S and the P/S solution at -20°C. Protect from light.